Intersegmental vessel Islet2 Nr2f1b Transcription factors Vascular development and zebrafish.Ĭopyright © 2022 Elsevier Inc. Together, we showed that the interaction of the Nr2f1b and Nr2f2 transcription factors in combination with the Islet2 play coordinated roles in the vascular development of zebrafish. Moreover, co-immunoprecipitation results indicated that Isl2 and Nr2f1b interact physically. In-vitro luciferase assay results, we showed that Isl2 and Nr2f1b can cooperatively enhance gene expression. We hypothsized that Isl2 and Nr2f1b can function together through cis-regulatory binding motifs. We found isl2 can regulate the expression of nr2f1b and nr2f2, suggesting a model where Isl2 functions upstream of Nr2f1b and Nr2f2. We further tested the genetic relationship among these three transcription factors. The double knockdown of isl2/nr2f1b, isl2/nr2f2, and nr2f1b/nr2f2 showed the enhanced defects in vasculature including less completed ISV, reduced veins, and ISV cells. In this study, we report that Nr2f2 plays minor roles on vein and intersegmental vessels (ISV) growth and dissect the genetic interactions among the three transcription factors Isl2, Nr2f1b, and Nr2f2 using a combinatorial knockdown strategy. However, the interaction between Isl2 and Nr2f1b is not known. We previously identified the transcription factors Islet2 (Isl2) and Nr2f1b required for specification of the vein and tip cell identity mediated by notch pathway in zebrafish. Electronic address: regulators controlling arterial identity are well described however, transcription factors that promote vein identity and vascular patterning have remained largely unknown. 6 Department of Biological Sciences, National Sun Yat-sen University, Taiwan Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University, Kaohsiung, Taiwan Doctoral Degree Program in Marine Biotechnology, Academia Sinica, Taipei, Taiwan Department of Biotechnology, Kaohsiung Medical University, Taiwan Institute of Medical Science and Technology, National Sun Yat-sen University, Taiwan.Electronic address: 1 Department of Biological Sciences, National Sun Yat-sen University, Taiwan Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University, Kaohsiung, Taiwan Doctoral Degree Program in Marine Biotechnology, Academia Sinica, Taipei, Taiwan. 5 Department of Biological Sciences, National Sun Yat-sen University, Taiwan Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University, Kaohsiung, Taiwan Doctoral Degree Program in Marine Biotechnology, Academia Sinica, Taipei, Taiwan Institute of Biomedical Sciences, National Sun Yat-sen University, Taiwan.4 Department of Biological Sciences, National Sun Yat-sen University, Taiwan.3 Department of Biological Sciences, National Sun Yat-sen University, Taiwan Department of Biotechnology, Kaohsiung Medical University, Taiwan.2 Department of Biological Sciences, National Sun Yat-sen University, Taiwan Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University, Kaohsiung, Taiwan.1 Department of Biological Sciences, National Sun Yat-sen University, Taiwan Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University, Kaohsiung, Taiwan Doctoral Degree Program in Marine Biotechnology, Academia Sinica, Taipei, Taiwan.
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